RNA splicing

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RNA Splicing

RNA splicing, a post-transcriptional process necessary to form a mature mRNA, was discovered in the late 1970s. Two different modes of splicing have been defined, that is, constitutive splicing and alternative splicing. Constitutive splicing is the process of removing introns from the premRNA, and joining the exons together to form a mature mRNA. Alternative splicing, on the other hand, is the ...

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RNA splicing

In the 20 years since the discovery that many eukaryotic genes are interrupted by non-coding intervening sequences, or introns, biologists have learnt a tremendous amount about the nuclear machinery that removes these introns from precursor gene transcripts before they are translated into proteins. This nuclear machinery must recognize the introns in the mRNA transcripts and then excise them by...

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Understanding splicing regulation through RNA splicing maps

Alternative splicing is a highly regulated process that greatly increases the proteome diversity and plays an important role in cellular differentiation and disease. Interactions between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing decisions. Findings from recent genome-wide studies of protein-RNA interactions have been combined with assays of the global effe...

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Visualizing RNA splicing in vivo.

Ribozymes are RNA molecules capable of associating with other RNA molecules through base-pairing and catalyzing various reactions involving phosphate group transfer. Of particular interest to us is the well known ribozyme from Tetrahymena thermophila capable of catalyzing RNA splicing in eukaryotic systems, chiefly because of its potential use as a gene therapy agent. In this article we review ...

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Split genes and RNA splicing.

A number of genes in higher organisms and in their viruses appear to be split. That is, they have "nonsense" stretches of DNA interspersed within the sense DNA. The cell produces a full RNA transcript of this DNA, nonsense and all, and then appears to splice out the nonsense sequences before sending the RNA to the cytoplasm. In this article what is known about these intervening sequences and ab...

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ژورنال

عنوان ژورنال: Current Biology

سال: 1998

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(98)00005-0